Review



mouse monoclonal anti rad21  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Novus Biologicals mouse monoclonal anti rad21
    Mouse Monoclonal Anti Rad21, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/Rad21+Antibody+(CM110-2C10)+-+BSA+Free/pmc09617616-155-66-72
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti rad21 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Activity-Induced Cortical Glutamatergic Neuron Nascent Proteins
    Article Snippet: Inputs and NeutrAvidin purified protein were run in a MiniPROTEAN electrophoresis cell (catalog #1658004, BIO-RAD; and compatible precast gels) and transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (catalog #1704150, BIO-RAD; and compatible transfer kits). .. The membranes were blocked with 5% blocking reagent (catalog #1706404, BIO-RAD) in TBST (136 mM NaCl, 20 mM Tris, pH adjusted to 7.0, 0.05% Tween 20) for 1 h at room temperature, incubated overnight at 4°C with the following primary antibodies in blocking buffer: polyclonal goat anti-biotin antibody (1:1000; catalog #31852, Thermo Fisher Scientific; RRID:AB_2096845); rabbit monoclonal anti-Smarca2 (1:1000; catalog #ab240648, Abcam); mouse monoclonal anti-Rad21 (1:1000; catalog #NB100-386, Novus Biologicals; RRID:AB_ 10000864); rabbit polyclonal anti-ARHGEF2 (1:1000; catalog #ab155785, Abcam; RRID:AB_2818944); rabbit monoclonal anti-NCAM (1:1000; catalog #NBP2-66 968, Novus Biologicals); and mouse monoclonal anti-ANHAK (1:1000; catalog #ab68556, Abcam; RRID:AB_1209218). .. Membranes were then washed with TBST three times for 10min and incubated in blocking buffer with the following secondary antibodies: rabbit-anti-goat conjugated with HRP (1:2500; catalog #172–1034, BIORAD; RRID:AB_11125144); goat anti-rabbit conjugated with HRP (1:2500; catalog #172–1019, BIO-RAD; RRID:AB_11125143; and goat anti-mouse conjugate with HRP (1:2500; catalog #172–1011, BIO-RAD; RRID:AB_11125936) for 2 h at room temperature.

    Article Title: Activity-Induced Cortical Glutamatergic Neuron Nascent Proteins
    Article Snippet: Inputs and NeutrAvidin purified protein were run in a Mini-PROTEAN electrophoresis cell (catalog #1658004, BIO-RAD; and compatible precast gels) and transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (catalog #1704150, BIO-RAD; and compatible transfer kits). .. The membranes were blocked with 5% blocking reagent (catalog #1706404, BIO-RAD) in TBST (136 m m NaCl, 20 m m Tris, pH adjusted to 7.0, 0.05% Tween 20) for 1 h at room temperature, incubated overnight at 4°C with the following primary antibodies in blocking buffer: polyclonal goat anti-biotin antibody (1:1000; catalog #31852, Thermo Fisher Scientific; RRID: AB_2096845 ); rabbit monoclonal anti-Smarca2 (1:1000; catalog #ab240648, Abcam); mouse monoclonal anti-Rad21 (1:1000; catalog #NB100-386, Novus Biologicals; RRID: AB_10000864 ); rabbit polyclonal anti-ARHGEF2 (1:1000; catalog #ab155785, Abcam; RRID: AB_2818944 ); rabbit monoclonal anti-NCAM (1:1000; catalog #NBP2-66 968, Novus Biologicals); and mouse monoclonal anti-ANHAK (1:1000; catalog #ab68556, Abcam; RRID: AB_1209218 ). .. Membranes were then washed with TBST three times for 10 min and incubated in blocking buffer with the following secondary antibodies: rabbit-anti-goat conjugated with HRP (1:2500; catalog #172–1034, BIO-RAD; RRID: AB_11125144 ); goat anti-rabbit conjugated with HRP (1:2500; catalog #172–1019, BIO-RAD; RRID: AB_11125143 ; and goat anti-mouse conjugate with HRP (1:2500; catalog #172–1011, BIO-RAD; RRID: AB_11125936 ) for 2 h at room temperature.

    Incubation:

    Article Title: Activity-Induced Cortical Glutamatergic Neuron Nascent Proteins
    Article Snippet: Inputs and NeutrAvidin purified protein were run in a MiniPROTEAN electrophoresis cell (catalog #1658004, BIO-RAD; and compatible precast gels) and transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (catalog #1704150, BIO-RAD; and compatible transfer kits). .. The membranes were blocked with 5% blocking reagent (catalog #1706404, BIO-RAD) in TBST (136 mM NaCl, 20 mM Tris, pH adjusted to 7.0, 0.05% Tween 20) for 1 h at room temperature, incubated overnight at 4°C with the following primary antibodies in blocking buffer: polyclonal goat anti-biotin antibody (1:1000; catalog #31852, Thermo Fisher Scientific; RRID:AB_2096845); rabbit monoclonal anti-Smarca2 (1:1000; catalog #ab240648, Abcam); mouse monoclonal anti-Rad21 (1:1000; catalog #NB100-386, Novus Biologicals; RRID:AB_ 10000864); rabbit polyclonal anti-ARHGEF2 (1:1000; catalog #ab155785, Abcam; RRID:AB_2818944); rabbit monoclonal anti-NCAM (1:1000; catalog #NBP2-66 968, Novus Biologicals); and mouse monoclonal anti-ANHAK (1:1000; catalog #ab68556, Abcam; RRID:AB_1209218). .. Membranes were then washed with TBST three times for 10min and incubated in blocking buffer with the following secondary antibodies: rabbit-anti-goat conjugated with HRP (1:2500; catalog #172–1034, BIORAD; RRID:AB_11125144); goat anti-rabbit conjugated with HRP (1:2500; catalog #172–1019, BIO-RAD; RRID:AB_11125143; and goat anti-mouse conjugate with HRP (1:2500; catalog #172–1011, BIO-RAD; RRID:AB_11125936) for 2 h at room temperature.

    Article Title: Activity-Induced Cortical Glutamatergic Neuron Nascent Proteins
    Article Snippet: Inputs and NeutrAvidin purified protein were run in a Mini-PROTEAN electrophoresis cell (catalog #1658004, BIO-RAD; and compatible precast gels) and transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (catalog #1704150, BIO-RAD; and compatible transfer kits). .. The membranes were blocked with 5% blocking reagent (catalog #1706404, BIO-RAD) in TBST (136 m m NaCl, 20 m m Tris, pH adjusted to 7.0, 0.05% Tween 20) for 1 h at room temperature, incubated overnight at 4°C with the following primary antibodies in blocking buffer: polyclonal goat anti-biotin antibody (1:1000; catalog #31852, Thermo Fisher Scientific; RRID: AB_2096845 ); rabbit monoclonal anti-Smarca2 (1:1000; catalog #ab240648, Abcam); mouse monoclonal anti-Rad21 (1:1000; catalog #NB100-386, Novus Biologicals; RRID: AB_10000864 ); rabbit polyclonal anti-ARHGEF2 (1:1000; catalog #ab155785, Abcam; RRID: AB_2818944 ); rabbit monoclonal anti-NCAM (1:1000; catalog #NBP2-66 968, Novus Biologicals); and mouse monoclonal anti-ANHAK (1:1000; catalog #ab68556, Abcam; RRID: AB_1209218 ). .. Membranes were then washed with TBST three times for 10 min and incubated in blocking buffer with the following secondary antibodies: rabbit-anti-goat conjugated with HRP (1:2500; catalog #172–1034, BIO-RAD; RRID: AB_11125144 ); goat anti-rabbit conjugated with HRP (1:2500; catalog #172–1019, BIO-RAD; RRID: AB_11125143 ; and goat anti-mouse conjugate with HRP (1:2500; catalog #172–1011, BIO-RAD; RRID: AB_11125936 ) for 2 h at room temperature.



    Similar Products

    90
    Merck & Co mouse monoclonal anti-rad21 53a303
    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
    Mouse Monoclonal Anti Rad21 53a303, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/rad21+53a303/pmc11624272-14-0-9
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti-rad21 53a303 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology mouse monoclonal anti rad21
    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
    Mouse Monoclonal Anti Rad21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/Rad21+Antibody/pm39487368-222-109-125
    Average 93 stars, based on 1 article reviews
    mouse monoclonal anti rad21 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    92
    Bethyl a3000 080a rad21 mouse mab
    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
    A3000 080a Rad21 Mouse Mab, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/Rad21+Recombinant+Monoclonal+Antibody/pm39271794-280-25-24
    Average 92 stars, based on 1 article reviews
    a3000 080a rad21 mouse mab - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    90
    Millipore mouse monoclonal anti-rad21
    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
    Mouse Monoclonal Anti Rad21, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/%CE%B3h2ax+antibody/pm37725510-60-28-35
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti-rad21 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Novus Biologicals mouse monoclonal anti rad21
    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
    Mouse Monoclonal Anti Rad21, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/Rad21+Antibody+(CM110-2C10)+-+BSA+Free/pmc09617616-155-66-72
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti rad21 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology mouse anti rad21 monoclonal antibody
    siRNA and Primers in This Study
    Mouse Anti Rad21 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/Rad21+Antibody/pmc09579331-282-77-83
    Average 93 stars, based on 1 article reviews
    mouse anti rad21 monoclonal antibody - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    90
    Millipore mouse monoclonal anti-scc1/rad21
    siRNA and Primers in This Study
    Mouse Monoclonal Anti Scc1/Rad21, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/anti+%CE%B2+actin/pm23548868-48-24-26
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti-scc1/rad21 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology monoclonal mouse anti rad21 h 12
    siRNA and Primers in This Study
    Monoclonal Mouse Anti Rad21 H 12, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+rad21/Rad21+Antibody/pm21901524-51-25-30
    Average 93 stars, based on 1 article reviews
    monoclonal mouse anti rad21 h 12 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of RAD21, in these four samples. Gray dots often overlap with black dots. .

    Journal: EMBO Reports

    Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

    doi: 10.1038/s44319-024-00303-6

    Figure Lengend Snippet: ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of RAD21, in these four samples. Gray dots often overlap with black dots. .

    Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

    Techniques: Flow Cytometry, Immunoprecipitation, Western Blot

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

    doi: 10.1038/s44319-024-00303-6

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

    Techniques: Recombinant, Sequencing, Reverse Transcription, SYBR Green Assay, Software, Isolation

    siRNA and Primers in This Study

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: cccDNA Surrogate MC-HBV–Based Screen Identifies Cohesin Complex as a Novel HBV Restriction Factor

    doi: 10.1016/j.jcmgh.2022.08.002

    Figure Lengend Snippet: siRNA and Primers in This Study

    Article Snippet: The commercial antibodies used for Western blot in this study were as follows: rabbit anti-SMC3 monoclonal antibody (1:2000, 5696; Cell Signaling Technology, Danvers, MA), rabbit anti-SMC1A monoclonal antibody (1:10,000, ab109238; Abcam, Cambridge, MA), rabbit anti-CTCF polyclonal antibody (1:4000, ab188408; Abcam), rabbit anti-HBc polyclonal antibody (1:2000, B0586; Dako, Copenhagen, Denmark), rabbit anti-HBx polyclonal antibody (1:2000, ab39716; Abcam), mouse anti-PDS5B monoclonal antibody (1:50, SC-81635; Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-PDS5A monoclonal antibody (1:50, SC-515263; Santa Cruz Biotechnology), mouse anti-RAD21 monoclonal antibody (1:50, SC-271601; Santa Cruz Biotechnology), mouse anti–β-actin monoclonal antibody (1:5000, 66009-I-Ig; Proteintech, Chicago, IL), mouse anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) monoclonal antibody (1:5000, 66004-I-Ig; Proteintech), mouse anti-Flag-tag monoclonal antibody (1:1000, M2; Sigma), and mouse anti-HA-tag monoclonal antibody (1:2000, ab130275; Abcam).

    Techniques: Sequencing